The Buffalo Bills have had cheerleaders throughout their history with the expectation of the 1966 season. Eight girls from Buffalo State College came together to form the Buffalo Bills cheerleaders from 1960 to 1965. The squad disbanded after 1965 and in 1967 the Buffalo Jills became the official cheerleaders for the Bills. However, the squad is not officially owned by the Bills or the NFL. Instead, they are sponsored by local radio stations in Buffalo. The Buffalo Jills also feature Ambassador Jills. These ambassadors are members of the squad who do not perform. Instead their job is to focus more on community outreach and social events.

The squad is also involved in their Junior Jill squad. In addition to cheerleaders, the Bills have Billy Buffalo as their official mascot. The Bills are also one of three teams in the NFL who have a marching band. The Attica High School Marching Band can be seen performing at Bills home games before kickoff.